nebnext first strand synthesis kit  (New England Biolabs)

Journal: Methods and Protocols

Article Title: A Simple, Rapid, and Effective Heparinase Protocol to Enable Nucleic Acid Study from Frozen Heparinized Plasma

doi: 10.3390/mps6060112

Figure Lengend Snippet: Experimental conditions for heparinase treatment.

Article Snippet: cfRNAs suspended in UltraPure™ DNase/RNase-Free distilled water were incubated with Bacteroides heparinase I (NEB, Cat# P0735L, Ipswich, MA, USA) enzyme, 10X heparinase buffer (supplied with the Bacteroides heparinase I enzyme, NEB, Cat# P0735L, Ipswich, MA, USA), RNAse inhibitor (Applied biosystem, Cat# N8080119, Carlsbad, CA, USA), and NEBNext First Strand Synthesis Reaction Buffer (NEB, Cat# E7770L, Ipswich, MA, USA) for 1 h at 37 °C in a thermomixer (Eppendorf™ Thermomixer™ R, 9.75 L × 8.75 W × 4.75 H (25 cm × 22 cm × 12 cm)).

Techniques: